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KMID : 0364820210570020091
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Korean Journal of Microbiology
2021 Volume.57 No. 2 p.91 ~ p.98
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Lead uptake capacity of thermophilic bacteria Aeribacillus pallidus strains isolated from Merapi volcano, Indonesia
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Rakhmawati Anna
Wahyuni Endang Tri
Yuwono Triwibowo
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Abstract
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Lead uptake capacity of four lead-tolerant thermophilic bacteriaAeribacillus pallidus strains MRP112, MRP148, MRP272, andMRP278, originally isolated from Merapi volcano, were examinedfor their potential as an absorbent to uptake lead (Pb) from anaqueous solution. The growth profile and Pb uptake capacitywere evaluated in Luria Bertani (LB) medium supplementedwith Pb 100 mg/L. The optimum condition was evaluated undervarious analytical parameters, including temperature (55°C,60°C, 65°C), pH (5, 6, 7), and contact time (6, 15, 24 h). Theinteraction of bacterial cell surface with ion Pb was provenusing Fourier transform infrared spectroscopy (FT-IR). Theoptimal growth of all strains was observed at 55°C and pH 7.
Aeribacillus pallidus MRP112 showed the highest uptake capacityof 97.41 ± 0.82 mg/g at 55°C, pH 5 for 15 h. FT-IR analysisdisplayed involved various functional groups such as hydroxyl,carboxyl, amine, and amide group ligands of protein in bindingPb ion. The A. pallidus strain represents a good candidate forthe bioremediation of Pb-contaminated environment.
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KEYWORD
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Aeribacillus pallidus, cell surface, growth curve, Pb-uptake, thermophilic bacteria
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